Improving pregnancy rate in IVF cycles by preparing sperm via microfluidic sperm chips
Effect of microfluidic sperm chips to sperm motility and DNA integrity improvement is already known by some studies. Although, microfluidic technologies were introduced in ART field at the beginning of 21st century and are being advanced from day to day, there is still no prevailing utilization in laboratories and there are few studies presenting their […]
ESHRE 2017: Selection of spermatozoa with higher chromatin integrity through a microfluidics device
Study question: We tested a simple method to enrich spermatozoa with higher progressive motility and superior chromatin status in men with normal and abnormal semen parameters. Summary answer: A microfluidics device was able to isolate spermatozoa with higher progressive motility and the lowest incidence of chromatin fragmentation in oligo- and normo-spermic semen. What is known […]
Alkaline and neutral Comet assay profiles of sperm DNA damage in clinical groups
The analysis of sperm DNA fragmentation has become a new marker to predict male infertility, and many techniques have been developed. The sperm Comet assay offers the possibility of differentiating single- and double-stranded DNA (ssDNA and dsDNA) breaks, which could have different effects on fertility. The objective of this study was to perform a descriptive […]
ESHRE 2018: Impact of microfluidic sperm sorting on embryo quality and comprehensive chromosome screening outcomes of couples with repeated implantation failure
What is the impact of the microfluidic sperm selection technique for IVF/ICSI on embryo quality and euploidy rates in couples with Repeated Implantation Failure (RIF)? When microfluid chip-sorted spermatozoa is used for IVF/ICSI, a higher number of top quality blastocysts and better euploidy rates were observed compared to standard selection. This was a retrospective analysis between 2016 […]
Double Stranded Sperm DNA Breaks, Measured by Comet Assay, Are Associated with Unexplained Recurrent Miscarriage in Couples without a Female Factor
It is known that sperm samples from recurrent pregnancy loss (RPL) couples have an increase in their sperm DNA fragmentation (SDF), but no studies have been performed in order to identify differences between single stranded SDF (ssSDF) and double stranded SDF (dsSDF) in these patients. This could be relevant because the type of DNA damage […]
Comprehensive analysis of sperm DNA fragmentation by five different assays: TUNEL assay, SCSA, SCD test and alkaline and neutral Comet assay
Sperm DNA fragmentation (SDF) is becoming an important test to assess male infertility. Several different tests are available, but no consensus has yet been reached as to which tests are most predictive of infertility. Few publications have reported a comprehensive analysis comparing these methods within the same population. The objective of this study was to […]
ASRM 2018: A Microfluidic Device for Selecting the Most Progressively Motile Spermatozoa Yields a Higher Rate of Euploid Embryos
Objective: We tested a novel approach for treating couples with complete and persistent embryo aneuploidy. Using a microfluidic device, we selected spermatozoa with the highest progressive motility and genomic integrity, capable of generating euploid embryos. Materials and Methods: Consenting men had their ejaculates screened by standard semen analysis according to WHO 2010 criteria. Specimens were […]
Poster SpermVD ESHRE 2017
Introduction The ability to cryopreserve a small number of human spermatozoa is essential in cases of severe male infertility, especially for patients requiring surgical sperm retrieval. Over the years, different methods for cryopreservation of small numbers of spermatozoa were proposed, however, each of them had drawbacks which prevented widespread use, mostly due to cumbersome preparation […]
Effects of the microfluidic chip technique in sperm selection for intracytoplasmic sperm injection for unexplained infertility: a prospective, randomized controlled trial.
PURPOSE: The new-generation spermatozoon selection method, microfluidic technique called Fertile Chip® gives the chance to select spermatozoa with lower DNA fragmentation indexes. We aimed to determine the effect of microfluidic techniques for spermatozoon selection in ICSI treatment in patients with unexplained infertility.
