Automation of human semen analysis using a novel artificial intelligence optical microscopic technology
Abstract Current semen analysis still commonly depends on a manual microscopy method in clinical laboratories worldwide. However, some of the major disadvantages of this technique are that it is labour‐intensive, subjective, laboratory‐based and time‐consuming. Although computer‐assisted semen analysers (CASAs) have enabled partialautomation of routine semen analysis, they lack wider acceptance due to their complicated operation. […]
A microfluidic sperm-sorting device reduces the proportion of sperm with double-stranded DNA fragmentation
La fragmentación del ADN espermático se puede producir en una (ssSDF) o en ambas (dsSDF) cadenas del ADN, estos daños están relacionados con dificultades para lograr un embarazo de forma natural y abortos espontáneos recurrentes, respectivamente. Las técnicas que se utilizan con más frecuencia para seleccionar espermatozoides requieren de centrifugación, lo que puede aumentar el […]
Optimizing the protocol for vitrification of individual spermatozoa by adjusting equilibration time
ABSTRACT: Efficient cryopreservation of small numbers of human spermatozoa is essential in cases of severemale infertility, especially those requiring surgical sperm retrieval. Although vitrifying individualspermatozoa on sperm vitrification devices (SpermVD®) provided optimal cell retrieval uponwarming, motility rates tended to be lower than with bulk-freezing. Post-warming motility isdirectly affected by cryoprotectant exposure; however, optimal cryoprotectant equilibration […]
Póster ASEBIR 2019: Utilización de la cámara microfluídica FERTILE en casos de fragmentación de la cadena doble del ADN de los espermatozoides
Webinar #3: MiOXSYS, Complementary evaluation for the study of male infertility
Continuando con el ciclo de seminarios organizado por CIMAB IBÉRICA, en el tercer seminario hablamos del estrés oxidativo y su diagnóstico mediante el sistema de análisis MiOXSYS. Tuvimos como invitados especiales al Sr. Bas Hersmus y el Professor Ralf Henkel, quienes compartieron sus conocimientos y experiencias en sus valiosas presentaciones. Si necesitas información adicional, no […]
A Comparison Between Two Assays for Measuring Seminal Oxidative Stress and Their Relationship With Sperm DNA Fragmentation and Semen Parameters
Abstract Oxidative stress (OS) is a significant cause of DNA fragmentation and is associated with poor embryo development and recurrent miscarriage. The aim of this study was to compare two different methods for assessing seminal OS and their ability to predict sperm DNA fragmentation and abnormal semen parameters. Semen samples were collected from 520 men […]
High Levels of Oxidation-Reduction Potential in Frozen-Thawed Human Semen Are Significantly Correlated With Poor Post-Thaw Sperm Quality
Abstract This study examined the relationship between oxidation-reduction potential (ORP) in frozen-thawed semen and the post-thaw sperm parameters. Levels of ORP were measured in 25 samples from men presenting for routine infertility work-up and were expressed as millivolt (mV)/106 sperm/ml. Frozen-thawed samples were examined for post-thaw total motility (TM%), progressive motility (PM%), total sperm count (TSC) […]
Oxidation-reduction Potential of Semen: What Is Its Role in the Treatment of Male Infertility?
Abstract The diagnosis of male infertility relies largely on conventional semen analysis, and its interpretation has a profound influence on subsequent management of patients. Despite poor correlation between conventional semen parameters and male fertility potential, inclusion of advanced semen quality tests to routine male infertility workup algorithms has not been widely accepted. Oxidative stress is […]
The Effect of Oxidative and Reductive Stress on Semen Parameters and Functions of Physiologically Normal Human Spermatozoa
Abstract Both oxidative stress (OS) and reductive stress (RS) are the two extreme facets of redox imbalance that can have deleterious effects on sperm function. However, there is a lack of information on the physiological range of oxidation-reduction potential (ORP). The aim of this study was to investigate the effect of OS and RS on […]
Clinical Relevance of Oxidation-Reduction Potential in the Evaluation of Male Infertility
Objective To evaluate (1) the relationship between oxidation-reduction potential (ORP) and abnormal sperm quality, and (2) the changes in ORP and sperm parameters over time, in search of a potential surrogate marker of poor sperm quality that may assist in the diagnosis of oxidative stress-related male infertility. Materials and Methods A total of 194 infertile […]
