A microfluidic sperm-sorting device reduces the proportion of sperm with double-stranded DNA fragmentation
La fragmentación del ADN espermático se puede producir en una (ssSDF) o en ambas (dsSDF) cadenas del ADN, estos daños están relacionados con dificultades para lograr un embarazo de forma natural y abortos espontáneos recurrentes, respectivamente. Las técnicas que se utilizan con más frecuencia para seleccionar espermatozoides requieren de centrifugación, lo que puede aumentar el […]
Póster ASEBIR 2019: FERTILE Chip vs Semen de Donante: Resultados clínicos en 101 ciclos de ICSI
Póster ASEBIR 2019: Efectividad de las técnicas de selección espermática en pacientes con fragmentación del ADN espermático alterada
Póster ASEBIR 2019: Utilización de la cámara microfluídica FERTILE en casos de fragmentación de la cadena doble del ADN de los espermatozoides
Alkaline and neutral Comet assay profiles of sperm DNA damage in clinical groups
The analysis of sperm DNA fragmentation has become a new marker to predict male infertility, and many techniques have been developed. The sperm Comet assay offers the possibility of differentiating single- and double-stranded DNA (ssDNA and dsDNA) breaks, which could have different effects on fertility. The objective of this study was to perform a descriptive […]
Double Stranded Sperm DNA Breaks, Measured by Comet Assay, Are Associated with Unexplained Recurrent Miscarriage in Couples without a Female Factor
It is known that sperm samples from recurrent pregnancy loss (RPL) couples have an increase in their sperm DNA fragmentation (SDF), but no studies have been performed in order to identify differences between single stranded SDF (ssSDF) and double stranded SDF (dsSDF) in these patients. This could be relevant because the type of DNA damage […]
Comprehensive analysis of sperm DNA fragmentation by five different assays: TUNEL assay, SCSA, SCD test and alkaline and neutral Comet assay
Sperm DNA fragmentation (SDF) is becoming an important test to assess male infertility. Several different tests are available, but no consensus has yet been reached as to which tests are most predictive of infertility. Few publications have reported a comprehensive analysis comparing these methods within the same population. The objective of this study was to […]
Double-stranded sperm DNA damage is a cause of delay in embryo development and can impair implantation rates
Comparative study of fertility parameters 2
Abstract The cryopreservation of spermatozoa has the main purpose of preserving male fertility. However, current preservation techniques have shown to produce lesions in the structure and alter sperm functions, probably due to the production of reactive oxygen species (ROS) during cryopreservation. To overcome the damage provoked by ROS, we introduced a novel antioxidant called EmbryORP® in […]
Comparative study of fertility parameters in vitrified human spermatozoa in the presence or absence of EmbryORP ® : A novel antioxidant
Abstract The cryopreservation of spermatozoa has the main purpose of preserving male fertility. However, current preservation techniques have shown to produce lesions in the structure and alter sperm functions, probably due to the production of reactive oxygen species (ROS) during cryopreservation. To overcome the damage provoked by ROS, we introduced a novel antioxidant called EmbryORP® in […]
