Alkaline and neutral Comet assay profiles of sperm DNA damage in clinical groups

  • Fecha: 1 marzo, 2012
  • Revista: Human Reproduction
  • Autores: J. Ribas-Maynou, A. García-Peiró, C. Abad, M.J. Amengual, J. Navarro, J. Benet

The analysis of sperm DNA fragmentation has become a new marker to predict male infertility, and many techniques have been developed. The sperm Comet assay offers the possibility of differentiating single- and double-stranded DNA (ssDNA and dsDNA) breaks, which could have different effects on fertility. The objective of this study was to perform a descriptive characterization of different groups of patients, such as those with asthenoteratozoospermic (ATZ) with or without varicocele, oligoasthenoteratozoospermic (OATZ) or balanced chromosome rearrangements, as compared with fertile donors. The Comet assay was used to investigate sperm samples for ssDNA and dsDNA breaks.