The study compares two sperm selection techniques in 239 couples undergoing IVF-ICSI: the traditional centrifugation gradient method and the microfluidic technique (ZyMot ICSI). Although there were no significant differences in fertilization rates (p = 0.87), the use of microfluidics improved the blastocyst rate (p = 0.046) and clinical pregnancy (p = 0.049). The results suggest that sperm selection using microfluidics could optimize embryo quality and pregnancy rates in assisted reproduction procedures.