{"id":3445,"date":"2025-02-20T14:13:58","date_gmt":"2025-02-20T13:13:58","guid":{"rendered":"https:\/\/fertiliberica.es\/publicacion-cientifica\/live-morphology-imaging-is-a-better-approach-for-observing-sperm-head-osmolality-induced-morphological-changes-compared-with-diff-quik-staining-method\/"},"modified":"2025-02-21T11:35:43","modified_gmt":"2025-02-21T10:35:43","slug":"live-morphology-imaging-is-a-better-approach-for-observing-sperm-head-osmolality-induced-morphological-changes-compared-with-diff-quik-staining-method","status":"publish","type":"publicacion","link":"https:\/\/fertiliberica.es\/pt-pt\/publicacion-cientifica\/live-morphology-imaging-is-a-better-approach-for-observing-sperm-head-osmolality-induced-morphological-changes-compared-with-diff-quik-staining-method\/","title":{"rendered":"Live Morphology Imaging is a Better Approach for Observing Sperm Head Osmolality-induced Morphological Changes Compared with Diff-Quik Staining Method"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">Objetctive<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">To compare the live morphology imaging technology of LensHooke\u00ae X1 PRO Semen Quality Analyzer with Diff-Quik staining in sperm head morphometric analysis.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Introduction<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">The dimensions of spermatozoa, especially head parameters, play a pivotal role for the sperm  morphology assessment according to WHO 5th manual for human sperm morphology assessment.<br>Diff-Quik is one of the staining methods recommended by the WHO manual. However, previous studies indicated that the hypotonic characteristics of Diff-Quik fixative and stain solutions might cause the spermatozoa to swell. Therefore, we compared the live morphology imaging technology of LensHooke\u00ae X1 Pro with the traditional Diff-Quik staining and microscopic live imaging in osmolality-induced morphological changes (OIMC) on spermatozoa.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Study design<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">After washed, sperms were resuspended either with or without 200 ppm HgCl2 in ddH2O, human tubal fluid (HTF) and 20% glucose respectively to generate hypo-, iso- and hyper-osmotic settng. Sperm head images were captured via a microscope (1000X, Olympus; BF for Diff-Quik; Ph3 annulus for live imaging) and the LensHooke\u00ae X1 PRO. The dimensions of head parameters i.e. length, width and area were  easured either by using the ImageJ software manually or by X1 PRO automatically. A total of 2000 individual sperms from 5 repeated tests were analyzed in each group. All analyses were conducted in a blinded fashion. Statistical analyses included t-test and two-way ANOVA with significance at p &lt; 0.01.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"http:\/\/cimabiberica.es\/wp-content\/uploads\/2021\/10\/image.png\" alt=\"\" class=\"wp-image-1343\"\/><\/figure>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large\"><img decoding=\"async\" src=\"http:\/\/cimabiberica.es\/wp-content\/uploads\/2021\/10\/image-1.png\" alt=\"\" class=\"wp-image-1344\"\/><\/figure><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Results<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Microscopic live imaging and X1 PRO showed minor changes in head area after ddH2O treatment (0.1%, p = 0.87; &#8211; 0.4%, p = 0.51). Hyper-osmotic exposure showed significant shrinkage (-11.5%, p &lt; 0.01; -12.3%, p &lt; 0.01) (Table 1). Head area were dramatically enlarged upon HgCl2 plus hypo-osmotic exposure in both microscopic live imaging and X1 PRO (9.4%, p &lt; 0.01; 8.0%, p &lt; 0.01) (Table 1). HgCl2 plus hyper-osmotic exposure showed no significant difference in both microscopic live imaging and X1 PRO (-0.4%, p = 0.49; -0.7%, p = 0.26) (Table 1). Diff-Quik method displayed inconsistent results of head area either with (hypo: -0.3%, p = 0.67, hyper: -23.1%, p &lt; 0.01) and without HgCl2 addition (hypo: -6.7%, p &lt; 0.01, hyper: -7.5%, p &lt; 0.01) compared with the other methods (Table 1, Fig. 3). X1 PRO took less analyzing time compared to microscopic live imaging (5.1 \u00b1 0.3 vs 319.3 \u00b1 27.4 min, n = 30, p &lt; 0.01 ), there was no significant difference between head morphometrics (p = 0.40) in contrast to Diff-Quik method (p &lt; 0.01). <\/p>\n\n\n\n<h2 class=\"wp-block-heading\">CONCLUSIONS <\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">LensHooke\u00ae X1 Pro live morphology imaging technology is fast and applicable in assessing OIMC. Diff-Quik staining method is not recommended for assessing sperm morphology when OIMC occurred.<\/p>\n","protected":false},"template":"","tags":[34],"producto":[91],"revista":[],"class_list":["post-3445","publicacion","type-publicacion","status-publish","hentry","tag-lenshooke","producto-lenshooke"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Live Morphology Imaging is a Better Approach for Observing Sperm Head Osmolality-induced Morphological Changes Compared with Diff-Quik Staining Method - Fertil Ib\u00e9rica<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/fertiliberica.es\/publicacion-cientifica\/live-morphology-imaging-is-a-better-approach-for-observing-sperm-head-osmolality-induced-morphological-changes-compared-with-diff-quik-staining-method\/\" \/>\n<meta property=\"og:locale\" content=\"pt_PT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Live Morphology Imaging is a Better Approach for Observing Sperm Head Osmolality-induced Morphological Changes Compared with Diff-Quik Staining Method - Fertil Ib\u00e9rica\" \/>\n<meta property=\"og:description\" content=\"Objetctive To compare the live morphology imaging technology of LensHooke\u00ae X1 PRO Semen Quality Analyzer with Diff-Quik staining in sperm head morphometric analysis. 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